Biodegradation potential of phosphate-mobilizing rhizobacteria Pseudomonas spp. towards glyphosate
Abstract
A series of in vitro experiments were conducted cultivating phosphate-mobilizing rhizobacteria in a modified Dworkin-Foster liquid nutrient medium with varying concentrations of herbicide as a phosphorus source. Planar (thin-layer) chromatography was used to study glyphosate biodegradation. Experimental data indicate that Pseudomonas spp. (P-6, P-7, P-11, P-15, P-42) rhizobacteria from the collection of the Institute of Soil Science and Agrochemistry metabolize glyphosate to safe sarcosine (methylglycine, Rf = 0,54 ± 0,022) and inorganic phosphate (Pi) without forming toxic aminomethylphosphonic acid. Using the data below on the inorganic phosphate (Pi) content in the cell-free culture fluid and taking into account the chemical composition of glyphosate, the destructive activity indices were calculated. At glyphosate concentrations of 300 mg/l, the destructive activity of Pseudomonas sp. P-6, Pseudomonas sp. P-7, Pseudomonas sp. P-11, Pseudomonas sp. P-15 and Pseudomonas sp. P-42 sources in some cases was 45,8–65,6 %. At glyphosate concentrations of 500 mg/l, the activity of GP destruction by strains of rhizobacteria Pseudomonas spp. (P-6, P-7, P-11, P-15, P-42) was within 43,1–50,1 % and was respectively: 43,1 %; 43,8 %; 48,4 %; 44,9 % and 50,1 %.
About the Authors
N. A. Mikhailouskaya
Institute of Soil Science and Agrochemistry
Belarus
Belarus
Minsk
S. S. Romanenko
Institute of Soil Science and Agrochemistry
Belarus
Belarus
Minsk
T. V. Pogirnitskaya
Institute of Soil Science and Agrochemistry
Belarus
Belarus
Minsk
T. B. Barashenko
Institute of Soil Science and Agrochemistry
Belarus
Belarus
Minsk
S. V. Dyusova
Institute of Soil Science and Agrochemistry
Belarus
Belarus
Minsk
A. L. Novik
Belarusian National Technical University
Belarus
Belarus
Minsk
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Review
For citations:
Mikhailouskaya N.A., Romanenko S.S., Pogirnitskaya T.V., Barashenko T.B., Dyusova S.V., Novik A.L. Biodegradation potential of phosphate-mobilizing rhizobacteria Pseudomonas spp. towards glyphosate. Soil Science and Agrochemistry. 2025;(2):66-75. (In Russ.)
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